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The change in pH of TSB and 1 PBS at different GSH concentrations was determined using a pH meter (Mettler-Toledo GmbH, Greifensee, Switzerland) calibrated with pH standards of 4.0 and 7.0 and the pH values of TSB and 1 PBS as a function of GSH concentration reported in Table 2

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IGF-1 LR3 exhibits approximately 10% of insulin's glucose-lowering potency due to structural similarity and weak insulin receptor binding