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Glutathione does protect hepatocytes from oxidative damage during lipid metabolism, but increasing glutathione levels won't override the hormonal signals (insulin resistance, leptin resistance) that drive fat accumulation in the first place

Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

Timothy Garvey, professor of medicine at the University of Alabama at Birmingham

(Never heard of oxidative stress