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However, regularly new mutations and variants of unknown significance (VUS) are identified, requiring functional studies such as enzyme activity measurements or cellular flux analysis for confirmation of the diagnosis and characterization [51]
K110-glutarylation and lipoyl glutarylation were detected in GCDH null cells, but the effects were variable, and GCDH depletion did not alter lipoylation or lysine glutarylation by immunoblot (Supplementary Figs
2 It also contributes to nitrogen balance in the body, which is vital for protein synthesis and healing
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