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Upregulation of -GCS-HS mRNA in Mucosa from TNBS-Treated Rats To examine if the partial recovery of the mucosal GSH levels observed between 12 weeks after TNBS+ethanol treatment was due to an upregulation of the rate limiting enzyme in the biosynthesis of GSH, we analyzed the -GCS-HS mRNA levels in mucosa from TNBS-treated rats

Supernatants were removed and treatments were stopped by the addition of 50 L lysis buffer containing PhosSTOP and Complete (Roche Diagnostics, Mannheim, Germany)

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Professor Alembick tells Tintin of his desire to visit Syldavia to research an ancient seal belonging to the Syldavian monarch King Ottokar IV