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Effect of Porcine gastric inhibitory polypeptide on beta-cell function in type I and type II diabetes mellitus
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Old methodology
IKK/ are specific activators of NF-B signaling although IKK can also activate both the AKT and mTOR kinases [78,79,80]
Blood samples in EDTA (for all analyses except glucagon, GLP-1 and glicentin) or P800 tubes (Becton Dickinson, Franklin Lakes, NJ) were immediately placed on ice and centrifuged at 4C and 2500g for 10 minutes, after which plasma was aliquoted and stored at -80C until analysis
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