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Retinas were then washed, refixed with 4% PFA for 20 min at RT and washed twice with PBS prior to mounting with Fluoromount-G (Southern Biotech, #0100-01)

Additionally, over 80% of patients in real-world settings stay on lower maintenance doses typically 1 mg or less for semaglutide and 7.5 mg or less for tirzepatide

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