After washing 3 times with PBS, neurons were incubated with 5.5 M pHluorin-labelled Syt1 nanobody in the presence of either 30mM KCl or 1M TTX (Alomone labs) to stimulate neurons or to silence them, respectively, for a total of 20 min at 37 C in a humid chamber
Environ Health Perspect, 95: 45-51
which can be enzymatically reduced back to GSH via NADPH-dependent glutathione reductase (GSR) activity [11]
In the gut, KPV enters epithelial cells through PepT1 transporters with a Km of roughly 160 uM, among the highest affinities reported for this transporter (Dalmasso et al
For example, in the brain, magnesium (Mg 2+ ) exerts anti-seizurogenic properties by antagonizing N-methyl-D-aspartate receptor (NMDAR)-excitatory activity (Ruppersberg et al., 1994)