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However, the combination of two distinct chemistries in one moiety such as in the GLP-1 analogues drastically impacts the extraction, chromatography and mass spectrometry performance

Graphical Abstract Similar content being viewed by others Introduction Metabolic dysfunction-associated steatotic liver disease (MASLD), with a steady upward trend in prevalence estimates from 25.3% in 19902006 to 38.2% in 20162019 [1], has become a leading cause of chronic liver disease worldwide [2,3,4], increasing the risk of both hepatic and extrahepatic complications, such as cardiovascular and chronic kidney diseases [5, 6]

Together with glucagon (GCG) , they form an integrated hormonal network that maintains glucose and energy balance

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