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A pertinent question arising from our findings is how SIRT3 facilitates macrophage phenotype transition through crosstalk with adipocytes

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Tissue was rinsed with ice-cold phosphate-based saline (PBS) to remove feces and then digested in Dulbeccos Modified Eagle Medium (DMEM) containing 10% FCS, 10 mg/100 ml collagenase A, 2 mg/100 ml Dispase II (both Roche/Sigma-Aldrich, Taufkirchen, Germany) and 7.7 mg/100 ml 1,4-Dithiothreit (Carl-Roth, Karlsruhe, Germany) for 10 min at 37 C