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Lower NAD+ affects how sirtuins work

The Keap1-Nrf2 pathway: mechanisms of activation and dysregulation in cancer

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The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)