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In addition, the N-terminal thioredoxin-like domain of GST, known as the G-site, is responsible for GSH binding 22

I ran a biochemical optimization program a while back, and one of the clients that I had in there, he tried methylene blue, and he only got up to a half a milligram or a milligram, and his mood was worse, his fatigue was worse, he had more anxiety, a bunch of problems that, you know, the dose was too low to say it was doing a pharmacological messing with his neurotransmitters

The reversible thiolation of proteins is known to regulate several metabolic processes including enzyme activity, transport activity, signal transduction and gene expression through redox-sensitive nuclear transcription factors such as AP-1, NF-kappaB (NF-kB) and p53 [21, 23]

Sanli, T., Steinberg, G

Int J Food Sci Technol