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Figure 1 2.3 Social interaction behavioral test Social behavior was evaluated using the three-chamber social test, a widely used method to assess social deficits in animal models of autism ( 2.4 Brain tissue collection and homogenization At the end of the experiment, the rats were euthanized by inhalation of an overdose of anesthetic (sevoflurane)

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Itching usually appears first, intensifying at night and disrupting sleep long before visible rashes fully develop