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It has been demonstrated that FOXO4-DRI can directly and with high affinity binds to the transactivation domain 2 (TAD2) of p53, competitively disrupting the FOXO4-p53 interaction within the nucleus

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Eur J Neurosci 20: 31793183

Essential means that the body cannot produce or synthesise these amino acids itself and must therefore take them in through food or appropriate dietary supplements