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A total of 20 g of protein per sample was separated using SDS-PAGE and electro-transferred onto Immuno-Blot PVDF Membrane (##1620177, Bio-Rad,

The one compound that appeared to reduce bacterial burden did so because infected macrophages were washed away after 24-h treatment, rather than through bactericidal activity against intracellular bacteria

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JAMA 310(24):26402649