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[PMC free article] [PubMed] [Google Scholar] Winterbourn CC, Brennan SO

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After harvesting, lysis was performed in buffer containing 50 mM Tris pH 7.6, 150 mM NaCl, and 0.5% NP-40, on ice via tissue grinding, and then proteasomes were isolated using M2 FLAG Affinity Gel (A2220