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They were maintained in IMDM medium (Invitrogen), and DMEM medium (Invitrogen) correspondingly, supplemented with 10% fetal bovine serum (FBS) (S711-001S, Lonsera) and 1% Penicillin-Streptomycin (SV30010, HyClone)

Key Takeaway: Chronic oxidative stress in the liver can lead to cellular damage and inflammation, making it harder for the organ to perform its 500+ vital functions

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If youre planning to combine intermittent fasting with GLP-1s, you need to do it intentionally with a smart nutrition strategy, consistent protein intake, and proper medical oversight