10.1371/journal.pone.0130353 300 ZhangY.NohK.SongW
Practices such as inadequate dosing, inappropriate treatment regimens, the free availability of drugs, management of patients by unqualified persons, and incomplete treatment courses have led to the development of subtherapeutic levels of antimony in the blood, promoting parasite tolerance and resistance to the drug (Kazemi-Rad et al., 2013)
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In brief, 10 L of powder extract (prepared by passing the slurry prepared in green solvent through a chromatographic column) was placed in a test tube along with 50 L of Ellmans reagent [50 mM of sodium acetate (NaoAc), 2 mM of 55-Dithio-bis-(2-nitobenzoic acid) in water, and 1M of Tris solution (pH 8.0)], 100 L of Tris solution, and 840 L of distilled water
Furthermore, the absence of strong data from clinical trials on novel therapeutic targets, such as inflammasome inhibitors or microbiome-modulating agents, limits the strength of conclusions regarding their efficacy and safety in humans