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Conclusion The current study enumerates a step-by-step design of experiments for the solubilization of IB proteins (probably of the non-classical type) for two amphipathic helices harboured on an AKAP (FAP65) that were surprisingly insoluble even after fusion with GST, a commonly used solubility-enhancer tag while producing recombinant proteins
What is glutathione reconstitution
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1g, Supplementary Fig
During phase II conjugation , glutathione binds to various toxins, medications, and metabolic waste products, rendering them water-soluble for excretion through bile or urine
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