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The study concluded on Day 9, showing oral administration of Niagen to be well tolerated in humans with no adverse effects. Third Human Niagen Study That Demonstrates Effectiveness: A study published in Nature Communications in 2018 shows that Niagen supplementation is well tolerated and effectively elevates NAD+ in a group of healthy middle-aged and older adults. The subject cohort was comprised of 60 healthy men and women between the ages of 55 and 79