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Syn-RA16 had a high binding affinity (Kd ~ 24.75 nM), akin to the previously documented RA16 aptamer, and displayed dose-dependent suppression of cell viability, with IC50 values around 118.4 nM

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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen, 0.5 mM L- glutamme, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

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