The template DNA was then digested with Dpn I and the plasmids were cloned in strain XL1-Blue
Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off
As an antioxidant enzyme with high expression in epididymal tissues, GPX5 plays an important role in maintaining the microenvironment of the epididymis, protecting sperm from oxidative stress, and maintaining the integrity of DNA structure
Oral supplements, on the other hand, face a tough journey
Then, for each of the six replicate determinations, the y intercept and the standard deviation of the y intercept were calculated