HEK293T cells (Cell Bank of Chinese Academy of Sciences, Shanghai, China) and human immortalized HSC cell line LX-2 (Procell Life Science & Technology, Wuhan, China) were cultured in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin and streptomycin (Gibco, USA) at a humidified 37 C incubator with 5% CO 2
SantamaraEAvilaMALatasaMURubioAMartn-DuceALuSCet alFunctional proteomics of nonalcoholic steatohepatitis: mitochondrial proteins as targets of S-adenosylmethionine
Hormone therapy replaces declining hormones directly
*Needless to handle needles, etc., operation is simple and painless
Exercise Regularly Regular physical activityboth aerobic and resistance trainingnaturally boosts GLP-1 production