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His fever and lymphadenitis persisted for 5 days and he also developed bilateral non-purulent conjunctivitis with peri-limbic sparing, erythematous and cracked lips, strawberry tongue and blanchable erythema over the trunk (Fig)
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After this, the resin was further washed with 25 CVs of buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 5 M tirzepatide, 0.03% (w/v) LMNG, 0.01% (w/v) glyco-diosgenin (GDN, Anatrace) and 0.008% (w/v) CHS
Then, the INT1-Se undergoes two successive [2,3]-sigmatropic rearrangements 44,45,46,47,48,49 to generate the para -amination intermediate ( INT3-Se ), which may readily react with HBr and then rearomatize to the desired product 2a (for details see Supplementary Figs 411)
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