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FLAG-tagged BRD4 transfected HEK293T cells were preincubated with the proteasomal pathway inhibitor (bortezomib) for 1 h to prevent BRD4 degradation

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Males ages 19-50 should consume at least 38 grams, while those 51 and older should aim for at least 30 grams

This does not reflect typical prevalence of migraine development in this age group and it is likely that controls who developed migraine were more inclined to participate in the second study visit due to the onset of migraine