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Recent advances in single-cell transcriptomics and spatial omics technologies have provided deeper insights into CSC-specific gene expression patterns, leading to the discovery of more refined CSC markers

Dont make insulin adjustments on your own consult your doctor for guidance

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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions