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The dried bacterial pellet was solubilized in 20 ml of B-PER (78243, Thermo Fisher Scientific), supplemented with protease inhibitors (1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l of benzonase) and 5 mM imidazole and then transferred to a 50-ml centrifuge tube

doi: 10.3389/fphar.2025.1526270 Received 11 November 2024 Accepted 28 January 2025 Published 17 February 2025 Volume 16 - 2025 Edited by Alessandra Linardi, Santa Casa de So Paulo School of Medical Sciences, Brazil Reviewed by David Pubill, University of Barcelona, Spain Ariadiny Lima Caetano, Santa Casa de So Paulo School of Medical, Brazil Updates Copyright 2025 Wang, Hao, Ma, Feng, Yang, An, Su and Feng