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These data suggest that EPACs are induced early in melanomagenesis and contribute to primary tumor growth by regulation of redox homeostasis, mitochondrial ROS, and glycolytic metabolism through activation of mTORC1-HIF-1-TXNIP signaling

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After neutralizing the mixture with NaOH it was subjected to ultracentrifugation through a membrane with a molecular weight cut off of 50,000 Da