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Samples were reconstituted into 3060 l sample solvent (water:methanol:acetonitrile, 2:1:1, v/v/v) and were centrifuged at 20,000 g at 4 C for 3 min

After that, they were exposed to 25 V for 20 min

Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)