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Inhaled exosomes or lipid nanoparticles (LNPs) functionalized with ligands for alveolar epithelial cell or macrophage-specific receptors (e.g., CD44 for macrophages) represent a promising strategy

4 Materials and methods 4.1 Cell culture and in vivo experiments 4.1.1 Cell lines Four human NB cell lines were used in this paper: Be(2)-C (MNA), IMR5-/75-shMYCN (29) (MNA, doxycycline-inducible shRNA targeting MYCN, resulting in MYCN downregulation), IMR-32 (MNA), and SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (non-MNA, doxycycline-inducible transgene, resulting in MYCN overexpression)

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SKINCARE THAT KEEPS ITS PROMISES Wash your face with a gentle cleanser and wait 15-30 minutes

The experiment requires dim lighting, and observers should maintain as much separation from the test animals as possible