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The main hypothesis for the physiopathology of takotsubo is that a rapid and significant increase of serum catecholamines (secondary to a stressful event: it was also known as the broken heart syndrome may cause microvascular coronary spasm, with inflammation and dysfunction (83)

Quantitative RT-PCR RNA isolation from human ESC and NPC cultures was done with the Aurum kit (Bio-Rad)

J Biol Chem (1998) 273(9):530714.10.1074/jbc.273.9.5307 47 RossRJLeungKCMaamraMBennettWDoyleNWatersMJet alBinding and functional studies with the growth hormone receptor antagonist, B2036-PEG (pegvisomant), reveal effects of pegylation and evidence that it binds to a receptor dimer