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Avocado is widely recognized as the fruit with the highest natural concentration of glutathione

To determine the molecular weight, the recombinant GHK peptides were dissolved in a 50 mM pH 7.4 Tris-HCl buffer at room temperature

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This pooled normal reference served as a common internal standard across experimental batches, enabling cross-batch normalization and direct quantitative comparison between polyp and gallstone samples while minimizing inter-batch technical variation