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These specimens were collected from the same cases as the frozen tissue biopsies described above (Table 1) and were used to assess protein ADP-ribosylation and oxidative stress (see the Measurement of oxidative stress section below) within the placenta for each of the three PE subclasses and controls

Relative Expression Software Tool (REST 2009, Corbett Research, Australia) was used to measure relative gene expression through the 2 -Ct approach

three random samples for each experimental condition were used for microarray analysis and all the four samples for quantitative polymerase chain reaction (qPCR) validation