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SNAP/FLAG-tagged wildtype (WT) or serine doublet mutant human GLP-1R constructs were expressed in INS-1 832/3 rat insulinoma cells with the endogenous rat receptor previously knocked out by CRISPR/Cas9 (11) (INS-1 832/3 GLP-1R KO cells), and WT or mutant receptors subsequently purified from these cells by immunoprecipitation under vehicle conditions or following a 5-minute stimulation with 100 nM GLP-1

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