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Controls were set up using comparable reaction conditions with shelterin and 300 M of the inactive control compound 60 or TRF1 only (without ligand)

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If you want a deeper dive into delivery systems, the liposomal delivery guide is a useful companion read

local use | Both | Local | Systemic | IGF-1 DES may be considered for intramuscular site-specific injection protocols by researchers studying local satellite-cell activation, whereas LR3 is used when prolonged systemic receptor engagement is the research objective [10, 11]

This is also why copying a figure from someone else rarely makes sense: their formulation, goals, and tolerability may differ from yours entirely