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Cells were centrifuged and resuspended in 0.1 M sodium cacodylate buffer and post-fixed for 1 h with 1% osmium + 0.1 M sodium cacodylate buffer, then dehydrated and embedded in Agar 100 resin

The column was Waters XBridge C8 column and gradient was from 20-90% MeCN/0.1% formic acid over 12 min