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To test the effect of Sp in paraquat-induced oxidative stress model, seven days old wild type flies, constitutively expressing mitochondrial (mt)-roGFP, were exposed to 10 mM paraquat for 24 h and then co-treated with 2.5 mM Sp for 24 h, prior to roGFP fluorescence estimation

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Spectroscopy Fluorescence excitation spectra were collected using a LS55 fluorescence spectrophotometer (Perkin Elmer Life Sciences, Five hundred microliter protein solution was placed in a 1 ml quartz cuvette