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The mixture containing: 1 FastStart Universal Probe Master (Rox) mix (Roche, Mannheim, Germany), 900 nM TaqMan forward and reverse primers and 250 nM of hydrolysis probe labeled with the fluorescent reporter dye FAM at the 5-end and a quenching dye at the 3-end and RNAse free water

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The production of ROS is increased in obesity due to the metabolic burden imposed by excessive macronutrient intake and the availability of substrates (43)

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