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The results generated by this methodology demonstrated that both PPO and PPO AC are responsive to riboflavin supplementation and PPO showed a strong dose-response effect

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Quantitative PCR assays were performed using 480 SYBR Green Master reagent mix (Roche Life Science, 4707516001, murine probes) or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science)

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Systematic rotation between injection sites prevents tissue changes (lipohypertrophy) that impair medication absorption and glucose control