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Fig.4 Efficacy evaluation of semaglutide and tirzepatide in HFD+CCl 4 -Induced MASH Mouse Models A

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Preparing for injection Pre-injection checklist: Reconstituted PT-141 refrigerated Insulin syringe (0.5-1ml, 29-31 gauge) Calculated dose volume Clean workspace Sharps container nearby Syringe selection: Insulin syringe: 0.5ml or 1ml capacity Needle gauge: 29-31 gauge (thin, less painful) Needle length: 5/16" to 1/2" (subcutaneous) Where to buy: Pharmacy, Amazon, medical supply No prescription needed in most areas Drawing the dose: Clean PT-141 vial stopper with alcohol wipe Let dry 30 seconds Insert syringe needle into vial Turn vial upside down (needle submerged) Pull plunger to desired volume (e.g., 0.3ml for 1.5mg) Check for air bubbles, tap syringe to remove Ensure accurate volume Remove from vial Injection site selection: Abdomen: Most common (2 inches from navel) Thigh: Front or side of thigh Upper arm: Back of upper arm (harder to reach) Rotate sites each injection Avoid same spot repeatedly Choosing injection site: Learn complete peptide injection technique at SeekPeptides

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