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RNA isolation, cDNA synthesis, and qRT-PCR analysis Total RNA was extracted from lettuce shoots and roots using TRIzol reagent (Invitrogene, USA) according to the manufacturers guidelines and clarified from DNA contamination utilizing DNase enzyme

Ipamorelin can stimulate the proliferation and differentiation of bone cells, promote the synthesis of bone matrix, thereby increasing bone mass and preventing the occurrence of osteoporosis

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On the other hand, the demonstration of paracetamol's progressive hepatic encephalopathy with severe seizures at least partly overruled the general discordance of NSAIDs' adverse effects and therapeutic application, paracetamol in particular as previously mentioned (1-10)

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