For each sample, 200 l of supernatant was added to 600 l of thiobarbituric acid (TBA) solution, reconstituted in glacial acetic acid, and then incubated at 95 C for 60 min in a dry-block thermostat (Bio TDB-100, Biosan, Latvia) and finally cooled immediately on ice for 10 min
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A
Moreover, the specificity of curcumins epigenetic actions remains uncertain
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