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Nitrosative stress leads to the overproduction of RNS, including nitric oxide (NO) which triggers an immune response, and its derivatives: nitrous acid (HNO 2 ), nitrogen dioxide (NO 2 ), and nitric acid (HNO 3 ) (91)

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Subsequently, clinical studies with pembrolizumab, another anti-PD-1 monoclonal antibody, in mCRPC patients also proved ineffective, indicating that monotherapy with PD-1 inhibitors in this patient population did not yield significant objective responses (Scheid et al., 2016)