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NCTC1469 cells, a normal mouse hepatocyte cell line, were obtained from the American Type Culture Collection and were cultured in DMEM containing 10% (v/v) fetal horse serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

A reduction in the amount of ferrozine-Fe 2 complex formed following antioxidant treatment indicates the presence of antioxidant chelators

Further insights into catalase structure : As mentioned above, only LSCs from clade 2 possess the short tetrapeptide gate interrupting the main channel from the surface of catalase down to the enzymes active sites

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