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found that Gln is a key amino acid in the regulation of glucose stability and insulin sensitivity, and the level of Gln affects the inflammatory response of skeletal muscle and regulates the expression of the adaptive protein GRB10, an insulin signaling inhibitor

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The cells were incubated overnight (approximately 16 h), and then 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)