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Watanabe, A., Okada, K., Shimizu, Y., Wakabayashi, H., Higuchi, K., Niiya, K.,

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The assay was carried out in the presence of mouse retinal lysate containing an enzyme buffer mixture (50 mM Tris-HCl [pH 7.4], 100 mM KCl, 5 mM MgCl 2 , 1 mM ADP, 0.5 mM PEP, and 0.2 mM NADH [reduced form of NAD + ]) and 8 U of LDH with a reaction volume of 1.0 ml

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