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The study was registered in ClinicalTrials.gov (Identifier: NCT04672447) and the protocol was approved by the Texas Tech University Institutional Review Board

The efficiency of biological activity is directly linked to the extraction method used, the solvents applied [31], the processing techniques such as bleaching or fermentation, and the conditions and age of the raw material [18,51], as well as the metabolomics of the specimen from which the leaves were obtained [11]

Amylin acts as a natural satiety signal that suppresses appetite, delays gastric emptying and reduces food intake by activating specific amylin receptors present in many regions of the brain

(2) stimulates insulin release and suppresses glucagon secretion in the pancreas

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