The Gila monster, a large venomous lizard native to the southwestern United States and Mexico, has given us not just a glimpse into the realm of animal toxins but also a groundbreaking medical advancement that many are now familiar with
That overlap is why many patients report their roughest days mid-to-late cycle rather than scattered randomly across the month
Proteins were clarified by centrifugation, quantified by BCA assay, reduced with DTT, alkylated with iodoacetamide, diluted and sequentially digested with LysC (1:50, 2 h) and trypsin (1:50, 14 h)
Clinical Endocrinology , 35(4), 405-415
The most frequent symptoms include nausea, vomiting, diarrhoea, abdominal pain, and constipation