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gentle swirling is sufficient if mixing is required Minimise the number of times the vial is accessed, as repeated needle punctures increase contamination risk Label the vial clearly with the date and time of reconstitution, and with the sponsor-validated beyond-use date Follow all additional handling instructions specified in the IMP label or protocol These measures help to slow the degradation processes described above

On day four, resazurin was added to a final concentration of 30 mol L 1 and fluorescence determined the next day

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