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The two elution fractions were mixed and dialyzed overnight in dialysis buffer 1 (20 mM TrisHCl (pH 8.0), 300 mM NaCl, 10% glycerol and 1 mM DTT) and 8 h in dialysis buffer 2 (20 mM TrisHCl (pH 8.0), 150 mM NaCl, 10% glycerol and 1 mM DTT)