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In mammalian cells, RNR activity requires GSH (Zahedi Avval & Holmgren, 2009

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Kit K006-F1 Kit K006-F5 Vial Part Number C021-1EA, Plastic vial C036-1EA, Glass vial Volume of DMSO to add per vial 1.5 mL 3.5 mL For # of Wells Up to 60 Up to 140 reaction Mixture Prepare the Reaction Mixture by vortexing the vials of Glutathione Reductase and NADPH Concentrates and then diluting one part each NADPH and Glutathione Reductase Concentrates 1:10 into eight parts Assay Buffer